SDS-PAGE vs. Western Blot - What's the Difference? | This vs. That

SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and Western Blot are two commonly used techniques in molecular biology for protein analysis. SDS-PAGE is a method that separates proteins based on their size using a gel matrix and an electric field. It allows for the visualization and quantification of proteins in a sample. On the other hand, Western Blot is a technique that combines SDS-PAGE with antibody detection to specifically identify and analyze a particular protein of interest. It involves transferring the separated proteins onto a membrane, followed by antibody binding and detection. While SDS-PAGE provides information about the size and relative abundance of proteins, Western Blot allows for the identification and characterization of specific proteins in a complex mixture. IntroductionSDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and Western Blot are two widely used techniques in molecular biology and biochemistry. They play crucial r...